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FATTY ACID PROFILING 

Essential Fatty Acids (EFA’s) [EFAs]are so termed, because of their indispensibility [indispensability]in biological (structural and metabolic) processes and for being a major source of energy. These are obtained from [the]diet because humans and animals cannot synthesize them. The two primary EFA’s [EFAs]4   that is lenoleic [linoleic]acid (Omega-6) and alpha lenoleic acid [alpha-linolenic acid]6 (Omega-3) are intimately involved in management of bodily inflammations [the management of body inflammations]7 . Fatty Acids are building blocks for prostaglandins which increase or decrease inflammation in the body. Most diets contain a great deal of fatty acid in the form of triacylglycerol (easters with glyerol) [esters with glycerol]8. Fatty acids comprise hydrocarbon chains terminating in carboxylic acid groups. Fatty acids and their associated derivatives are the primary components of and are responsible for the hydrophobic properties (insoluble in water) of lipids.

Specialized [Fatty Acid] Testing [Service] by [from] EFRAC

• Butyric acid (C4:0) • Caproic acid (C6:0) • Caprylic acid (C8:0) • Capric acid (C10:0) • Undecanoic acid (C11:0) • Lauric acid C12:0) • Tridedacnoic acid (C13:0) • Myristic acid ( 14:0) • Myristoleic acid ( 14:1) • Pentadecanoic acid ( 15:0)

Fatty Acid Profiling

Services

PARAMETERS

Saturated Fatty Acid:
• Butyric Acid (C4:0) • Caproic Acid (C6:0) • Caprylic Acid (C8:0) • Capric Acid (C10:0) • Undecanoic Acid (C11:0) • Lauric Acid (C12:0) • Tidecanoic Acid (C13:0) • Myristic Acid (C14:0) • Pentadecanoic Acid (C15:0) • Palmitic Acid (C16:0) • Heptadecanoic Acid (C17:0) • Stearic Acid (C18:0) • Arachidic Acid (C20:0) • Henicosanoic Acid (C21:0) • Behanic Acid (C22:0) • Tricosanoic Acid (C23:0) • Lignoceric Acid (C 24:0)

Mono-Unsaturated Fatty Acid:
• Myristoleic Acid (C14:1) • Cis-10-Pentadecanoic Acid (C15:1) • Palmitoleic Acid ( C16:1 ) • Cis-10-Heptadecanoic Acid ( C17:1 ) • Elaidic Acid (C18:1, Trans), Oleic Acid (C18:1) • Eicosenoic Acid (C20:1) • Erucic Acid ( C22:1) • Nervonic Acid (C24:1)

Poly-Unsaturated Fatty Acid:

• Linolelaidic Acid ( C18:2, Trans) • Linoleic Acid (C18:2, W6) • Y- Linolenic Acid ( C18:3) • Linolenic Acid (C18:3, W-3) • Cis -11-14- Eicosadienoic Acid (C20:2) • Cis-8,11,14-Eicosatrienoic Acid (C20:3n6) • Cis-11,14,17 – Eicosatrienoic Acid (C20:3n3) • Arachidonic Acid (C20:4n6) • Cis -13,16- Docosadienoic Acid (C22:2) • Eicosapentaenoic Acid (C20:5n3) • Nervonic Acid (C24:1) • Cis- 4,7,10,13,16,19 – Docosahexaenoic Acid (C22:6) Trans Fatty Acid: Elaidic Acid (C18:1) • Linolelaidic Acid ( C18:2)

Omega – 3

• Fatty Acid: Linolenic Acid ( C18:3) • Eicosapentaenoic Acid (C20:5n3) • Eicosapentaenoic Acid (C20:5n3)

Omega – 6

• Fatty Acid: Linolelaidic Acid ( C18:2, Trans) • Linoleic Acid (C18:2) • Y- Linolenic Acid ( C18:3) • Arachidonic Acid (C20:4n6)

Omega – 9
• Fatty Acid: Elaidic Acid (C18:1, Trans) • Oleic Acid (C18:1) • Erucic Acid ( C22:1) • Erucic Acid ( C22:1)

METHODS & GUIDELINES

• AOAC
• AOCS
• IS
• ISO
• USP
• FCC
• FSSAI Lab [Laboratory]Manual

INSTRUMENTS

• GC-FID

• HPLC-ELSD

• GC-MS/MS